A functional category list of proteins (Sparassis crispa) identified by 1-DGE and nESI-LC-MS/MS
The table lists indicated fraction numbers, matching peptides, scores, accesion numbers, protein names, theoretical mass (kDa), theoretical pH left to right.
Fraction Number	Matching Peptide	Score	Accession Number	Protein Name	Analytical MW	Theortical pI	Peptide
Chromatin structure and dynamics
6	1	92	gi\|1742929	Histone H2B *	15165.40	10.16	AMAILNSFVNDIFER
6	1	67	gi\|3142	Histone H4*	11366.34	11.36	TVTALDVVYALK
DNA replication, recombination, and repair
2	1	57	gi\|14245691	Reverse transcriptase *	40781.01	9.06	QILADLK
3	1	69	gi\|908896	DNA repair protein rad32*	73688.98	5.48	QINLLVK
5	1	47	gi\|47132515	recQ family Helicase*	169673.76	8.04	LEAGTAHDSSDGVGGGSAPAAPGK
Cell division and chromosome partitioning
4	1	102	gi\|10241488	Cell division control protein 10 *	38657.99	7.20	STLINTIFASHLIDSK
4	1	51	gi\|6977953	Septin*	53737.59	5.41	VNIIPIIAK
Transcription
4	1	49	gi\|57228114	Transcriptional activator*	133338.53	8.14	EEGEGLGEEGK
4	1	47	gi\|19068759	Heat shock transcription factor *	33685.36	6.62	EDLLGFDDSLR
Translation, ribosomal structure and biogenesis
1	3	208	gi\|58758727	Translation elongation factor EF1-a *	44184.90	8.55	EHALLAFTLGVR, TLLDAIDAIEPPVRPSDKPLRLPLQDVYK, YAWVLDKLK, YAWVLDK, AGMIVTFAPTNVTTEVK
1	3	193	gi\|11078214	Translation elongation factor EF1-a	46634.83	8.79	IGGIGTVPVGR, AGMIVTFAPTNVTTEVK, FMVTVIDAPGHR
3	5	263	gi\|2897607	Translation elongation factor EF1-a	50133.84	9.21	EHALLAFTLGVR, TLLDAIDAIEPPVRPSDKPLRLPLQDVYK, QTVAVGIIK, YAWVLDKLK, YAWVLDK
3	5	259	gi\|58758727	Translation elongation factor EF1-a	44184.90	8.55	EHALLAFTLGVR, TLLDAIDAIEPPVRPSDKPLRLPLQDVYK, YAWVLDKLK, YAWVLDK, AGMIVTFAPTNVTTEVK
3	4	237	gi\|2313	Translation elongation factor EF1-a	49828.51	9.12	QLIVAINK, EHALLAFTLGVR, YAWVLDKLK, YAWVLDK
3	2	149	gi\|32567511	Translation elongation factor EF1-a	14692.27	6.95	TLLDAIDANEPPVRPSDKPLR, EHALLAFTLGVR
3	1	71	gi\|8927046	Translation elongation factor EF2*	89733.93	6.75	GTVAFGSGLHGWAFTVR
5	1	59	gi\|46431916	Ribosomal protein L3*	43948.70	10.26	HGSLGFLPR
5	1	50	gi\|3766376	Ribosomal protein L10 *	33565.30	4.71	TSFFQALGIPTK
6	2	109	gi\|16943769	Ribosomal protein S19*	14522.61	9.48	LEVPTWVDLVK, ELAPYDPDWYYVR
6	1	81	gi\|3806	Translation initiation factor 5A-2*	17114.28	4.81	VHLVAIDIFTGK
6	1	56	gi\|3560198	Ribosomal protein L18E *	21189.79	11.66	AGGEVLTLDQLALR
6	1	56	gi\|11595555	Ribosomal protein S28*	15909.58	10.60	VSGVGLLALWK
6	1	53	gi\|1164943	Ribosomal protein S7e*	21987.65	9.73	ILEDLVFPTEIVGK
6	1	42	gi\|1850540	Ribosomal P2 phosphoprotein*	11145.20	4.17	DINELIASGPEK
Posttranslational modification, protein turnover, chaperones
2	4	271	gi\|7521943	Heat shock protein 70 *	71055.79	5.02	IINEPTAAAIAYGLDKK, TQDLLLLDVAPLSLGIETAGGVMTALIK, SINPDEAVAYGAAVQAAILSGDTSEK, IINEPTAAAIAYGLDK
2	1	66	gi\|19069227	26S Proteasome regulatory subunit 4*	47377.75	6.39	TMLELLNQLDGFDTR
2	1	65	gi\|56199674	60 kDa Chaperonin*	19860.09	5.99	GFISPYFITDVK
3	2	160	gi\|7521943	Heat shock protein 70	71055.79	5.02	IINEPTAAAIAYGLDKK, TQDLLLLDVAPLSLGIETAGGVMTALIK, SINPDEAVAYGAAVQAAILSGDTSEK, IINEPTAAAIAYGLDK
4	1	56	gi\|7521943	Heat shock protein 70	71055.79	5.02	IINEPTAAAIAYGLDKK, TQDLLLLDVAPLSLGIETAGGVMTALIK, SINPDEAVAYGAAVQAAILSGDTSEK, IINEPTAAAIAYGLDK
5	1	60	gi\|172714	Heat shock protein 70	16141.27	5.42	FELSGIPPAPR
Energy production and conversion
2	1	50	gi\|4376093	Pyruvate kinase *	58248.74	5.88	GDLGIEIPASQVFLAQK
3	1	45	gi\|4376093	Pyruvate kinase	58248.74	5.88	GDLGIEIPASQVFLAQK
3	2	140	gi\|584806	ATP synthase alpha chain*	59522.39	9.07	STVAQLVKTLEENDAMK, TGEIVDVPVGPELLGR
3	1	79	gi\|44985523	F0F1-type ATP synthase*	58941.62	9.29	TAVALDTILNQK
3	1	76	gi\|5190	Phosphoglycerate kinase *	44406.05	6.15	DGAITNNNR
3	1	53	gi\|2654	Vacuolar ATPase subunit b *	57199.76	4.96	TIFESLDLAWSLLR
3	1	52	gi\|469103	Fumarase *	53156.73	6.84	SLQNFDIGGPTER
4	2	130	gi\|46444820	Malate dehydrogenase *	34728.38	5.72	DDLFNTNASIVR, VAVLGAGGGIGQPLSLLLK
4	1	77	gi\|4029338	Malate dehydrogenase *	33029.29	6.13	VAVLGAAGGIGQPLSLLLK
4	1	81	gi\|46433360	NAD-formate dehydrogenase *	41801.55	5.51	LLGTVENELGIR
4	1	74	gi\|13785197	Inorganic pyrophosphatase *	32390.49	5.24	VLGIMALLDEGETDWK
4	1	45	gi\|218041	Phosphoglycerate kinase *	44656.34	8.14	ALESPQRPFLAILGGAK
Carbohydrate transport and metabolism
1	1	44	gi\|929983	Glycerol-3-aldehyde dehydrogenase *	30768.23	6.13	VPTNDVSVVDLVVR
1	1	42	gi\|5441844	Polygalacturonase 1*	38063.31	6.53	FFYAHSLDDSTITGLNVK
2	1	56	gi\|15808971	Glycogen synthase *	80847.52	6.03	GVDMFIESLAR
2	1	88	gi\|6651233	Trehalose phosphorylase *	83653.35	6.38	IINEPTAAAIAYGLDK
2	1	51	gi\|171569	1,4-glucan-6-(1,4-glucano)-transferase*	81129.57	5.76	LPAWITR
3	1	95	gi\|169852	Glyceraldehyde-3-phosphate dehydrogenase*	36055.25	7.01	VPTLDVSVVDLVVR
3	1	74	gi\|3367647	Enolase *	46877.80	5.15	LGANAILGVSLAVAKAGAAEK
3	1	47	gi\|40713647	Glucose-6-phosphate isomerase*	61445.79	6.38	ILLSNFFAQPEALAFGK
4	2	175	gi\|30038515	Glyceraldehyde-3-phosphate dehydrogenase	36607.76	7.67	VPTIDVSVVDLVVR, AVGNNIIPSSTGAAK
4	2	152	gi\|929979	Glycerol-3-phosphate dehydrogenase *	30895.28	5.71	VPTLDVSVVDLVVR, KVIISAPSSDAPMFVCGVNLDAYDPK
4	1	59	gi\|3289019	Xylose reductase *	36021.33	5.77	WAAQRGIAVIPK
4	1	44	gi\|19068698	Alpha alpha trehalase *	76692.14	5.92	LFEVVEGKRSK
5	1	65	gi\|68429	Triose-phosphate isomerase *	27234.99	6.13	IVIAYEPVWAIGTGK
5	1	42	gi\|19068698	Alpha alpha trehalase	76692.14	5.92	LFEVVEGKRSK
6	1	74	gi\|169852	Glyceraldehyde-3-phosphate dehydrogenase	36055.25	7.01	VPTLDVSVVDLVVR
Amino acid transport and metabolism
3	1	97	gi\|16415894	Aspartyl-proteinase*	18517.90	5.77	VILDTGSSNLWVPSTK
3	1	51	gi\|44980853	Aminopeptidase I *	53590.96	6.08	FFAGFLTDWR
Nucleotide transport and metabolism
1	1	49	gi\|44981739	Carbamoylphosphate synthase *	248131.78	5.48	AASTVDEALEAVK
1	1	45	gi\|22095310	C1-tetrahydrofolate synthase *	67745.49	6.28	AGCVNLKKHIQNAK
Lipid metabolism
2	1	43	gi\|4959943	Esterase *	170506.56	4.67	DLHLADWDGDGACDIIWTDPDNLNR
3	1	43	gi\|12718322	C-8, 7 sterol isomerase*	30464.65	6.51	AGDVGGGLLEKVVR
4	1	47	gi\|44986286	Diacylglycerol kinase *	63153.68	5.37	SSIISVDGENFPFEPIQVEVLPR
5	1	42	gi\|5725410	Phosphocholine cytidylyltransferase *	41556.61	5.39	FFYAHSLDDSTITGLNVK
Inorganic ion transport and metabolism
3	1	44	gi\|2776	Catalase *	57848.89	6.42	LFSYPDTHR
Cytoskeleton
3	2	137	gi\|508701	Actin *	41736.68	5.17	SYELPDGQVITIGNER, TTGIVLDSGDGVTHTVPIYEGFSLPHAILR
Cell envelope biogenesis, outer membrane
6	1	60	gi\|46442658	GPI-protein transamidase *	55466.46	5.40	EQTNVKDLIVK
6	1	44	gi\|51215619	Chitin biosynthesis protein *	27327.64	5.31	K.NGLRLGKIPK.A
Intracellular trafficking and secretion
6	1	48	gi\|2072023	GABA-receptor-associated protein*	22479.01	6.66	YLVPSDLTVGQFVYVIR
Signal transduction
4	5	314	gi\|11262436	14-3-3 protein homolog *	28913.33	4.67	QAFDDAIAELDTLSEESYKDSTLIMQLLR, AASDVAVTELPPTHPIR, QAFDDAIAELDTLSEESYK, DSTLIMQLLR, YLAEFATGDK
5	1	53	gi\|3218407	Adenylate cyclase*	190334.33	5.86	ELNIANNK
Unclassified
1	1	53	gi\|6321879	Protein involved in RNA metabolism	12009.47	5.05	IEEVIDLILR
2	1	52	gi\|6321879	Protein involved in RNA metabolism	12009.47	5.05	IEEVIDLILR
4	3	179	gi\|30024660	guanine nucleotide binding protein	34556.86	5.98	DGITMLWDLNEGK, GWVTAIATSSENPDMILTASR, LWDLNTGLTTR
4	1	44	gi\|28564107	SOL1	16776.24	5.58	FAGRDSVK
5	1	67	gi\|46438514	ATP/ADPtranslocator	32754.91	9.62	EFNGLVDVYKK
Asterisks indicate unique proteins identified from S. crispa fruiting body using Tandem MS analysis and Edman sequencing. Commonly identified proteins in S. crispa and H. erinaceum are shown in red letters.